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Pathway analysis
OBJECTIVE
- Protein pathway analysis based on Aracyc
was applied to the phosphoproteins. Our objective is to find out whether protein phosphorylation is rich in some particular pathways.
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METHOD:
- To utilize Aracyc,
we first found the othologs of the oilseed rape phosphoproteins in TAIR7 by reciprocal BLAST with e-value cutoff of 10-10.
- All the locus ID of the othologs were then upload to Aracyc online service
to map the proteins to pathways in Aracyc.
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RESULT:
- By reciprocal BLAST (e-vlaue: 10-10), we found 2244 orthologs in Arabidopsis thaliana (TAIR7).
- Mapping these 2244 orthologs to Aracyc, 600 genes can be found in Aracyc database,
which is shown in this page.
- Result shows that phosphorylation is rich in some pathways such as Glycolytic pathway, Sucrose degradation pathway, Calvin cycle and some other more.
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