Protein categorization

OBJECTIVE

  • Protein categorization based on GO slim was applied to the phosphoproteins. Our objective is to find out whether protein phosphorylation is rich in some function categories.

METHOD:

  • To utilize TAIR GO annotation, we first found the othologs of the oilseed rape phosphoproteins in TAIR7 by reciprocal BLAST with e-value cutoff of 10-10.
  • All the locus ID of the othologs were then upload to TAIR GO online service to get the Protein categorization on GO cellular component, GO biological process and GO molecular function.

RESULT:

  • By reciprocal BLAST (e-vlaue: 10-10), we found 2244 orthologs in Arabidopsis thaliana (TAIR7).
  • Using these 2244 orthologs, the Protein categorizations are shown in this page.
  • By comparing the Protein categorization of the 2244 orghologs to the Protein categorization of the whole arabidopsis genome, we found that phosphorylation is rich in some function categories such as protein binding, kinase activity in molecular function, DNA or RNA metabolism in biological process, Plastid in cellular component and some others.
 

© 2008 Thelen lab and Xu lab at University of Missouri.